8DPW
The structure of Interleukin-11 Mutein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.230, 37.097, 68.532 |
| Unit cell angles | 90.00, 101.31, 90.00 |
Refinement procedure
| Resolution | 33.600 - 1.800 |
| R-factor | 0.1966 |
| Rwork | 0.195 |
| R-free | 0.22710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mhl |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.630 |
| Data reduction software | Aimless |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.101 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.083 | 1.374 |
| Rpim | 0.043 | 0.719 |
| Number of reflections | 12637 | 761 |
| <I/σ(I)> | 11.8 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 6.9 |
| CC(1/2) | 0.990 | 0.603 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 27% PEG 3350, 0.1 M bis-tris propane pH 9, 0.2 M ammonium sulfate, 5 mM praseodymium chloride |
