8DEY

Ternary complex structure of Cereblon-DDB1 bound to IKZF2(ZF2,3) and the molecular glue DKY709

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9999
Spacegroup name	P 1 21 1
Unit cell lengths	96.476, 96.227, 155.598
Unit cell angles	90.00, 93.46, 90.00

Refinement procedure

Resolution	84.150 - 3.700
R-factor	0.3011
Rwork	0.298
R-free	0.34800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7u8f
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	84.150	84.150	3.800
High resolution limit [Å]	3.700	16.550	3.700
Rmerge	0.151	0.056	2.108
Rmeas	0.169	0.064	2.352
Total number of observations	152319
Number of reflections	30254	341	2215
<I/σ(I)>	5.92	20.08	0.76
Completeness [%]	98.8	90	99.9
Redundancy	5.035	4.097	5.257
CC(1/2)	0.996	0.993	0.307

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	1.6 M potassium/sodium phosphate, pH 6.5