8D6J
Human Ago2 bound to miR122(21nt) with PIWI loop swapped to AtAgo10 sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2021-10-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.302, 107.512, 68.706 |
| Unit cell angles | 90.00, 106.90, 90.00 |
Refinement procedure
| Resolution | 39.220 - 2.500 |
| R-factor | 0.2343 |
| Rwork | 0.232 |
| R-free | 0.28410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ola |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.220 | 2.589 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.037 | 0.296 |
| Rmeas | 0.419 | |
| Rpim | 0.296 | |
| Number of reflections | 30141 | 2992 |
| <I/σ(I)> | 12.9 | 2.58 |
| Completeness [%] | 98.8 | 99.17 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.832 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1M Tris 8, 0.01 MgCl2, 0.1M Phenol,15% PEG 3350, 10% Isoproponal. |
