8CZ9
Crystal Structure of the E372K LNK SH2 Domain mutant in Complex with a JAK2 pY813 Phosphopeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.95373 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 75.829, 37.618, 50.497 |
Unit cell angles | 90.00, 108.76, 90.00 |
Refinement procedure
Resolution | 35.900 - 1.650 |
R-factor | 0.1877 |
Rwork | 0.185 |
R-free | 0.21630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7r8w |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.900 | 1.700 |
High resolution limit [Å] | 1.640 | 1.650 |
Rmeas | 0.760 | |
Number of reflections | 111266 | 11000 |
<I/σ(I)> | 20.45 | 2.49 |
Completeness [%] | 99.0 | |
Redundancy | 6.8 | |
CC(1/2) | 0.990 | 0.818 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 281.15 | 18% PEG 8000, 0.05 M MgAc , 0.1 M Tris ph 8.5 |