8CZ8

Novel Anti-Mesothelin Antibodies Enable Crystallography of the Intact Mesothelin Ectodo- main and Engineering of Potent, T cell-engaging Bispecific Therapeutics

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.78652
Spacegroup name	P 32
Unit cell lengths	83.795, 83.795, 230.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.160 - 2.600
R-factor	0.1918
Rwork	0.190
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f3f
RMSD bond length	0.003
RMSD bond angle	1.280
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.093	0.697
Number of reflections	55600	2751
<I/σ(I)>	30.8
Completeness [%]	99.9
Redundancy	4.7
CC(1/2)	0.988	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	295	bicine (9.0), glycerol, ammonium sulfate