8CUH
Crystal structure of human TEAD2 complexed with its inhibitor TM2.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.071, 62.285, 79.914 |
| Unit cell angles | 90.00, 117.70, 90.00 |
Refinement procedure
| Resolution | 38.925 - 2.400 |
| R-factor | 0.1864 |
| Rwork | 0.184 |
| R-free | 0.23460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hgu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.099 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmeas | 0.820 | |
| Rpim | 0.030 | 0.460 |
| Number of reflections | 20774 | 863 |
| <I/σ(I)> | 24.1 | 1.5 |
| Completeness [%] | 97.0 | 83.7 |
| Redundancy | 3.6 | |
| CC(1/2) | 0.720 | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.1 M Hepes, pH 7.2, 2.4 M sodium formate. |
