8CUH

Crystal structure of human TEAD2 complexed with its inhibitor TM2.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	124.071, 62.285, 79.914
Unit cell angles	90.00, 117.70, 90.00

Refinement procedure

Resolution	38.925 - 2.400
R-factor	0.1864
Rwork	0.184
R-free	0.23460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hgu
RMSD bond length	0.008
RMSD bond angle	1.099
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmeas		0.820
Rpim	0.030	0.460
Number of reflections	20774	863
<I/σ(I)>	24.1	1.5
Completeness [%]	97.0	83.7
Redundancy	3.6
CC(1/2)	0.720	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	0.1 M Hepes, pH 7.2, 2.4 M sodium formate.