8CGZ
tubulin-AB8939 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-17 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.692, 128.760, 253.204 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 126.600 - 2.527 |
| R-factor | 0.1992 |
| Rwork | 0.197 |
| R-free | 0.23500 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 126.600 | 2.760 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Rmeas | 0.145 | |
| Rpim | 0.040 | 0.521 |
| Number of reflections | 47709 | 1871 |
| <I/σ(I)> | 12.7 | 1.5 |
| Completeness [%] | 66.5 | |
| Redundancy | 13.1 | 13.1 |
| CC(1/2) | 0.996 | 0.354 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | PEG, LiSO4, PIPES BUFFER, pH 6.80 |
