8CAF

N8C_Fab3b in complex with NEDD8-CUL1(WHB)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	102.372, 106.871, 180.651
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	90.330 - 2.660
R-factor	0.2189
Rwork	0.217
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.177
Data reduction software	XDS (Version November 3, 2014)
Data scaling software	Aimless
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	180.000	2.740
High resolution limit [Å]	2.660	2.660
Rmerge	0.055	1.165
Number of reflections	56730	4609
<I/σ(I)>	23.8
Completeness [%]	98.6
Redundancy	6.7
CC(1/2)	0.999	0.612

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2.0-2.1M Ammonium Sulfate, 0.1M Citrate pH 6.0, 10mM TCEP