8C8J

Long Interspersed Nuclear Element 1 (LINE-1) reverse transcriptase ternary complex with hybrid duplex and dTTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-05
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9762
Spacegroup name	C 1 2 1
Unit cell lengths	119.349, 84.528, 107.938
Unit cell angles	90.00, 91.48, 90.00

Refinement procedure

Resolution	59.660 - 2.100
R-factor	0.2093
Rwork	0.208
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.890
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.11.8 (8-JUN-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.660	2.150
High resolution limit [Å]	2.100	2.100
Rpim	0.018	0.427
Number of reflections	62715	4676
<I/σ(I)>	18.7	1.9
Completeness [%]	100.0	100
Redundancy	41.2	42.1
CC(1/2)	1.000	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	PEG8000, sodium citrate, 1,4 dioxane, ethylene glycol, dimethyl sulphoxide
2	VAPOR DIFFUSION	5.6	293	PEG8000, sodium citrate, 1,4 dioxane, ethylene glycol, dimethyl sulphoxide
3	VAPOR DIFFUSION	5.6	293	PEG8000, sodium citrate, 1,4 dioxane, ethylene glycol, dimethyl sulphoxide
4	VAPOR DIFFUSION	5.6	293	PEG8000, sodium citrate, 1,4 dioxane, ethylene glycol, dimethyl sulphoxide
5	VAPOR DIFFUSION	5.6	293	PEG8000, sodium citrate, 1,4 dioxane, ethylene glycol, dimethyl sulphoxide
6	VAPOR DIFFUSION	5.6	293	PEG8000, sodium citrate, 1,4 dioxane, ethylene glycol, dimethyl sulphoxide