8C0F

Tubulin-PTC596 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-14
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.999995
Spacegroup name	P 21 21 21
Unit cell lengths	104.680, 158.260, 180.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.550 - 2.100
R-factor	0.1956
Rwork	0.194
R-free	0.22520
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.550	49.550	2.160
High resolution limit [Å]	2.100	9.390	2.100
Rmerge	0.138	0.019	3.965
Rmeas	0.144	0.019	4.122
Total number of observations	2378365
Number of reflections	174197	2135	12635
<I/σ(I)>	15.97	101.29	0.7
Completeness [%]	99.7	98.6	98.6
Redundancy	13.653	12.572	13.28
CC(1/2)	0.999	1.000	0.259

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	3% PEG 4K, 3% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine