8BX2
Oct4/Sox2 protein:DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97624 |
| Spacegroup name | P 63 |
| Unit cell lengths | 163.169, 163.169, 39.792 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 81.720 - 3.140 |
| R-factor | 0.20075 |
| Rwork | 0.199 |
| R-free | 0.25234 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ht5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.508 |
| Data reduction software | XDS (20200417) |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | MOLREP (11.7.03) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.585 | 3.342 |
| High resolution limit [Å] | 3.137 | 3.137 |
| Rmerge | 0.114 | 2.135 |
| Number of reflections | 8506 | 424 |
| <I/σ(I)> | 14.8 | 1.6 |
| Completeness [%] | 77.4 | 22.7 |
| Redundancy | 19.5 | 18.4 |
| CC(1/2) | 0.996 | 0.548 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M HEPES, 70% (v/v) MPD |
