8BQ7

Structure of the mouse 8-oxoguanine DNA Glycosylase mOGG1 in complex with ligand TH2829

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	80.733, 82.095, 169.487
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.993 - 2.600
Rwork	0.255
R-free	0.30690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7pz1
RMSD bond length	0.002
RMSD bond angle	0.653
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.993	2.720
High resolution limit [Å]	2.600	2.600
Number of reflections	35462	4247
<I/σ(I)>	11.4
Completeness [%]	100.0
Redundancy	13.4
CC(1/2)	0.998	0.589

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	10 % w/v PEG4000, 20 % glycerol, 0.02 M monosaccharide, 0.1 M bicine/Trizma base pH 8.5