8AY1
Crystal structure of the C. elegans POFUT2 (CePoFUT2) triple mutant (R298K-R299K-A418C) in complex with the Rattus norvegicus TSR4 single mutant (E10C) from F-spondin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 63 |
| Unit cell lengths | 156.848, 156.848, 80.451 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.900 - 2.130 |
| R-factor | 0.19531 |
| Rwork | 0.194 |
| R-free | 0.24037 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5foe |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.389 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.240 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rpim | 0.765 | |
| Number of reflections | 62973 | 8995 |
| <I/σ(I)> | 10.6 | 1.1 |
| Completeness [%] | 99.6 | 98.1 |
| Redundancy | 8.5 | 8.1 |
| CC(1/2) | 0.998 | 0.491 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | BIS-Tris calcium chloride dihydrate MPD ammonium sulfate |
