8AY1

Crystal structure of the C. elegans POFUT2 (CePoFUT2) triple mutant (R298K-R299K-A418C) in complex with the Rattus norvegicus TSR4 single mutant (E10C) from F-spondin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	GDP-fucose protein O-fucosyltransferase 2,Spondin-1	polymer	462	53320.2	2	UniProt (Q8WR51) UniProt (Q3B7D6) Pfam (PF10250) Pfam (PF00090) UniProt (by SIFTS) (P35446)	Caenorhabditis elegans	Patterning defective protein 2,Peptide-O-fucosyltransferase 2,O-FucT-2,F-spondin
2	C, P (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
3	D, E, F, G, H... (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	11	Chemie (EDO)
4	AA, BA, J, K, L... (B, A)	SULFATE ION	non-polymer		96.1	12	Chemie (SO4)
5	Q (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	CA, DA (A, B)	water	water		18.0	155	Chemie (HOH)

Sequence modifications

A, B: 39 - 424 (UniProt: Q8WR51)

PDB	External Database	Details
Lys 298	Arg 298	engineered mutation
Lys 299	Arg 299	engineered mutation
Cys 418	Ala 418	engineered mutation
Ser 979	-	linker
Ser 980	-	linker
Gly 981	-	linker
Gly 982	-	linker
Gly 983	-	linker
Gly 984	-	linker
Ser 985	-	linker
Gly 986	-	linker
Gly 987	-	linker
Gly 988	-	linker
Gly 989	-	linker
Ser 990	-	linker
Gly 991	-	linker
Gly 992	-	linker
Gly 993	-	linker
Gly 994	-	linker
Gly 995	-	linker
Ser 996	-	linker
Ser 997	-	linker
Gly 998	-	linker
Ser 999	-	linker
Ser 1000	-	linker

A, B: 1001 - 1054 (UniProt: Q3B7D6)

PDB	External Database	Details
Cys 1010	Glu 622	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	106640.3
Non-Polymers*	Number of molecules	26
	Total formula weight	2370.0
All*	Total formula weight	109010.3

*Water molecules are not included.