8AX8
Human Apolipoprotein E4 (ApoE4) N-terminal domain (space group P3121)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-27 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 47.693, 47.693, 104.773 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.425 - 1.551 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gs9 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.300 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.063 | |
| Number of reflections | 20733 | 991 |
| <I/σ(I)> | 17.3 | |
| Completeness [%] | 99.8 | |
| Redundancy | 9.5 | |
| CC(1/2) | 0.999 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | HEPES, PEG3350 |
