8ACF

Structure of the argX-117 in complex with a complement C2 fragment at low pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	71.830, 89.140, 146.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.570 - 1.800
R-factor	0.1788
Rwork	0.178
R-free	0.19850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ani
RMSD bond length	0.014
RMSD bond angle	1.112
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.570	1.864
High resolution limit [Å]	1.800	1.800
Rmerge	0.069	2.457
Number of reflections	87785	8651
<I/σ(I)>	19.2
Completeness [%]	100.0	99.98
Redundancy	13.4	13.6
CC(1/2)	1.000	0.472

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	50% MPD, 100 mM Hepes pH 5.5-6.0