7ZYH

Crystal structure of human CPSF30 in complex with hFip1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-15
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.2809
Spacegroup name	P 1 21 1
Unit cell lengths	60.127, 115.125, 66.444
Unit cell angles	90.00, 116.78, 90.00

Refinement procedure

Resolution	48.650 - 2.200
R-factor	0.2498
Rwork	0.247
R-free	0.26440
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.008
Data reduction software	XDS (2019-03-15)
Phasing software	autoSHARP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.650	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	226720	15294
<I/σ(I)>	13.53
Completeness [%]	92.3
Redundancy	6
CC(1/2)	0.998	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292.15	1.626 M (NH4)SO4, 0.1 M Bis-Tris pH 6.5