7ZVN
Crystal structure of human Annexin A2 in complex with full phosphorothioate 5-10 2'-methoxyethyl DNA gapmer antisense oligonucleotide solved at 1.87 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.621, 57.502, 70.478 |
Unit cell angles | 90.00, 90.24, 90.00 |
Refinement procedure
Resolution | 43.750 - 1.870 |
R-factor | 0.1688 |
Rwork | 0.167 |
R-free | 0.19810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lpu |
RMSD bond length | 0.009 |
RMSD bond angle | 1.026 |
Data reduction software | XDS (v3) |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.750 | 1.990 |
High resolution limit [Å] | 1.870 | 1.870 |
Number of reflections | 36723 | 35540 |
<I/σ(I)> | 12.14 | |
Completeness [%] | 99.8 | |
Redundancy | 6.6 | |
CC(1/2) | 1.000 | 0.720 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 290 | 4% w/v PEG 4000, 14% v/v glycerol, 0.03 M MgCl2/CaCl2, 0.1 M bicine/Trizma base pH 8.3 |