7ZRB
Crystal structure of Beta-catenin Armadillo repeats domain in complex with the inhibitor RS6452
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.880, 160.240, 79.970 |
| Unit cell angles | 90.00, 99.63, 90.00 |
Refinement procedure
| Resolution | 45.264 - 3.434 |
| R-factor | 0.2359 |
| Rwork | 0.233 |
| R-free | 0.28330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1th1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.653 |
| Data reduction software | XDS |
| Data scaling software | SAINT |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.840 | 3.660 |
| High resolution limit [Å] | 3.430 | 3.430 |
| Rmerge | 0.219 | 1.882 |
| Number of reflections | 12910 | 646 |
| <I/σ(I)> | 7.3 | 1 |
| Completeness [%] | 90.8 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.996 | 0.358 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 7-9% (w/v) PEG 6000, 100 mM Na Citrate, pH 5.6, 5 mM DTT, 15% (v/v) Isopropanol |
