7ZOZ
Crystal structure of Siglec-15 in complex with Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-11 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.97925 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 216.534, 60.525, 53.373 |
Unit cell angles | 90.00, 100.82, 90.00 |
Refinement procedure
Resolution | 106.341 - 2.104 |
R-factor | 0.1778 |
Rwork | 0.176 |
R-free | 0.21820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vkk |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 106.342 | 2.180 |
High resolution limit [Å] | 2.104 | 2.104 |
Rmerge | 0.078 | 0.633 |
Rmeas | 0.084 | 0.707 |
Rpim | 0.033 | 0.307 |
Number of reflections | 37172 | 1824 |
<I/σ(I)> | 15.1 | |
Completeness [%] | 99.8 | |
Redundancy | 6.5 | |
CC(1/2) | 0.998 | 0.835 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20 % PEG 3350 0.2 M Calcium Chloride |