7ZOG
Crystal structure of Cryptosporidium parvum -Plasmodium falciparum mutant lysyl tRNA synthetase in complex with inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97628 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.103, 118.732, 143.454 |
Unit cell angles | 90.00, 90.69, 90.00 |
Refinement procedure
Resolution | 64.900 - 1.800 |
R-factor | 0.183 |
Rwork | 0.182 |
R-free | 0.21000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6hcw |
RMSD bond length | 0.009 |
RMSD bond angle | 1.452 |
Data reduction software | DIALS (3.4.3-1) |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.900 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.084 | 0.991 |
Number of reflections | 226134 | 1234 |
<I/σ(I)> | 11 | 1.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.5 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 300 | Reservoir: 0.1 M tris, pH 7.8, 0.2 M lithium sulfate, 25% PEG 3350 |