7ZMO
Crystal structure of human RECQL5 helicase APO form in complex with engineered nanobody (Gluebody) G3-052
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9119 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 89.517, 150.870, 269.342 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.670 - 3.750 |
| Rwork | 0.283 |
| R-free | 0.30630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lb5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.794 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.670 | 3.080 |
| High resolution limit [Å] | 3.030 | 3.030 |
| Number of reflections | 71635 | 3558 |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | |
| CC(1/2) | 1.000 | 0.100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1M ammonium sulfate -- 0.5% PEG8000 -- 0.1M HEPES pH 7.0 |
