7ZMN

Crystal structure of human RECQL5 helicase APO form in complex with engineered nanobody (Gluebody) G3-048

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9119
Spacegroup name	P 2 2 21
Unit cell lengths	79.503, 89.573, 259.056
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	129.528 - 3.200
Rwork	0.242
R-free	0.27460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lb5
RMSD bond length	0.006
RMSD bond angle	1.540
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	129.630	2.790
High resolution limit [Å]	2.740	2.740
Number of reflections	49418	2406
<I/σ(I)>	4.8
Completeness [%]	99.6
Redundancy	6.6
CC(1/2)	1.000	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2M ammonium sulfate -- 0.1M HEPES pH 7.5