7ZMN
Crystal structure of human RECQL5 helicase APO form in complex with engineered nanobody (Gluebody) G3-048
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9119 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 79.503, 89.573, 259.056 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 129.528 - 3.200 |
Rwork | 0.242 |
R-free | 0.27460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lb5 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.540 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 129.630 | 2.790 |
High resolution limit [Å] | 2.740 | 2.740 |
Number of reflections | 49418 | 2406 |
<I/σ(I)> | 4.8 | |
Completeness [%] | 99.6 | |
Redundancy | 6.6 | |
CC(1/2) | 1.000 | 0.400 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2M ammonium sulfate -- 0.1M HEPES pH 7.5 |