7ZLY

Crystal structure of human GPCR Niacin receptor (HCA2) expressed from Spodoptera frugiperda

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 21 2 21
Unit cell lengths	81.080, 82.150, 86.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.970 - 2.700
Rwork	0.250
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pxz
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.972	2.752
High resolution limit [Å]	2.700	2.700
Number of reflections	25559	1101
<I/σ(I)>	10.18
Completeness [%]	95.8
Redundancy	5.6
CC(1/2)	0.990	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	100mM pH5.4 sodium citrate, 60mM ammonium citrate, 36% PEG400, and 3% Additive 80 (40% PPG)