7ZKS

SRPK1 IN COMPLEX WITH INHIBITOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99989
Spacegroup name	P 65 2 2
Unit cell lengths	74.877, 74.877, 309.168
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.310 - 2.280
R-factor	0.2455
Rwork	0.243
R-free	0.28970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xv7
RMSD bond length	0.007
RMSD bond angle	0.900
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER (2.11.8 (8-JUN-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.850	2.530
High resolution limit [Å]	2.280	2.280
Rmeas	0.059	0.586
Number of reflections	23552	6045
<I/σ(I)>	19.84	2.52
Completeness [%]	95.9	94.9
Redundancy	4.2	4.4
CC(1/2)	0.999	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	5 mM HEPES pH 7.4, 150 mM NaCl, 20 mM DTT