7ZFB

SARS-CoV-2 Omicron RBD in complex with nanobody C1, Omi-18 and Omi-31 Fabs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-04
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	111.188, 135.092, 112.244
Unit cell angles	90.00, 101.66, 90.00

Refinement procedure

Resolution	70.560 - 3.080
R-factor	0.2629
Rwork	0.261
R-free	0.30780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7qnw
RMSD bond length	0.004
RMSD bond angle	0.679
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.000	3.130
High resolution limit [Å]	3.080	3.080
Rmerge	0.264
Rpim	0.073
Number of reflections	60199	2994
<I/σ(I)>	5.4	0.2
Completeness [%]	100.0
Redundancy	14.1
CC(1/2)	0.997	0.286

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Ammonium sulfate, 0.1 M MES pH 6.5, 20 % w/v PEG 8000