7ZDD
Crystal structure of TRIM33 PHD-Bromodomain isoform B in complex with H3K10ac histone peptide.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 197 |
Detector technology | PIXEL |
Collection date | 2021-07-05 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.130, 52.893, 105.698 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.380 - 1.625 |
R-factor | 0.2099 |
Rwork | 0.208 |
R-free | 0.25020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mr8 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.860 |
Data reduction software | autoPROC (1.1.7 (20200918)) |
Data scaling software | STARANISO (Version 2.3.46 (17-Sep-2020) |
Phasing software | PHASER (2.8.3) |
Refinement software | BUSTER (2.11.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.385 | 1.776 |
High resolution limit [Å] | 1.625 | 1.625 |
Rmerge | 0.098 | 1.184 |
Rpim | 0.043 | 0.462 |
Number of reflections | 16564 | 829 |
<I/σ(I)> | 12 | 1.6 |
Completeness [%] | 92.2 | 57.7 |
Redundancy | 6.2 | 7.3 |
CC(1/2) | 0.998 | 0.595 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295.15 | 0.2 M ammonium chloride, 0.1 M sodium acetate pH 5.0, 20% w/v PEG 6000 |