7ZAN
Crystal Structure of human IL-17A in complex with IL-17RA and IL-17RC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 186.305, 186.305, 238.305 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.550 - 5.061 |
| R-factor | 0.2969 |
| Rwork | 0.294 |
| R-free | 0.35310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hsa 6hg4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.850 |
| Data reduction software | XDS (Version Feb. 5, 2021) |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 133.601 | 133.601 | 5.078 |
| High resolution limit [Å] | 5.061 | 23.449 | 5.061 |
| Rmerge | 0.103 | 0.027 | 6.272 |
| Rmeas | 0.106 | 0.028 | 6.436 |
| Rpim | 0.025 | 0.009 | 1.437 |
| Total number of observations | 1872 | 1940 | |
| Number of reflections | 10707 | 135 | 97 |
| <I/σ(I)> | 15.8 | 81.8 | 0.5 |
| Completeness [%] | 100.0 | 96.4 | 100 |
| Redundancy | 19.2 | 13.9 | 20 |
| CC(1/2) | 0.999 | 0.999 | 0.404 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M MES, 10.0% PEG DME 500 |
