7ZA1
GPC3-Unc5D octamer structure and role in cell migration
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-22 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91587 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.643, 157.584, 126.601 |
| Unit cell angles | 90.00, 102.95, 90.00 |
Refinement procedure
| Resolution | 97.336 - 4.100 |
| Rwork | 0.324 |
| R-free | 0.36480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ftt hGPC3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.572 |
| Data reduction software | autoPROC |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 123.400 | 4.550 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Rmerge | 0.109 | |
| Rmeas | 0.130 | |
| Rpim | 0.071 | |
| Number of reflections | 22459 | 900 |
| <I/σ(I)> | 3.4 | 1.6 |
| Completeness [%] | 73.0 | 10.9 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.994 | 0.738 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 291.15 | 20% ethylene glycol, 10% w/v PEG 8000, 0.1 M Tris/BICINE (pH 8.5) and 0.02 M of amino acids (L-Na-glutamate, alanine, glycine, lysine-HCl, and serine) |
