7ZA1

GPC3-Unc5D octamer structure and role in cell migration

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (E, F, G, H)	Netrin receptor UNC5D	polymer	268	30258.0	4	UniProt (F1LW30) Pfam (PF25609) Pfam (PF07679) Pfam (PF00090)	Rattus norvegicus (Norway rat)	Protein unc-5 homolog D
2	E, F, G, H (A, C, D, B)	Glypican-3	polymer	464	53383.7	4	UniProt (P51654) Pfam (PF01153)	Homo sapiens (human)	GTR2-2,Intestinal protein OCI-5,MXR7
3	I (I)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
4	J, K, L, M (J, K, M, N)	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	4	In PDB GlyTouCan (G81315DD)
5	AA, BA, CA, DA, EA... (A, C, D, B, E...)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	12	Chemie (NAG)
6	P, Q, S, T, W... (E, F, H)	alpha-D-mannopyranose	non-polymer		180.2	6	Chemie (MAN)

Sequence modifications

E, F, G, H: 46 - 307 (UniProt: F1LW30)

PDB	External Database	Details
His 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag
His 313	-	expression tag

A, C, D, B: 32 - 483 (UniProt: P51654)

PDB	External Database	Details
Glu 29	-	expression tag
Thr 30	-	expression tag
Gly 31	-	expression tag
Phe 359	Ser 359	variant
Gly 484	-	expression tag
Thr 485	-	expression tag
Lys 486	-	expression tag
His 487	-	expression tag
His 488	-	expression tag
His 489	-	expression tag
His 490	-	expression tag
His 491	-	expression tag
His 492	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	334566.6
Branched	Number of molecules	5
	Total formula weight	3419.1
Non-Polymers*	Number of molecules	18
	Total formula weight	3735.4
All*	Total formula weight	341721.1

*Water molecules are not included.