7Z2M
Crystal Structure of the 11.003 Fab in complex with human IL-17A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999802 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.996, 107.335, 269.297 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 134.649 - 1.899 |
| R-factor | 0.2026 |
| Rwork | 0.201 |
| R-free | 0.22730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hr9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS (Jan 31, 2020) |
| Data scaling software | Aimless (CCP4-7.0) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | BUSTER (2.11.7 (20-MAY-2020)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 134.649 | 134.649 | 1.905 |
| High resolution limit [Å] | 1.899 | 8.811 | 1.899 |
| Rmerge | 0.130 | 0.034 | 2.780 |
| Rmeas | 0.138 | 0.036 | 2.930 |
| Rpim | 0.045 | 0.011 | 0.913 |
| Total number of observations | 20244 | 20359 | |
| Number of reflections | 190056 | 2150 | 1869 |
| <I/σ(I)> | 11.1 | 37.2 | 1.1 |
| Completeness [%] | 98.8 | 99.7 | 98.2 |
| Redundancy | 10.2 | 9.4 | 10.9 |
| CC(1/2) | 0.998 | 0.999 | 0.327 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2M sodium potassium tartrate, 20% (w/v) PEG 3,350 |
