7Z26

Crystal structure of YTHDF2 YTH domain in complex with m6A RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-28
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 65
Unit cell lengths	80.690, 80.690, 114.270
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.340 - 1.900
R-factor	0.1989
Rwork	0.197
R-free	0.23020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rdn
RMSD bond length	0.008
RMSD bond angle	1.058
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.340	2.010
High resolution limit [Å]	1.900	1.900
Number of reflections	33384	5365
<I/σ(I)>	20.97
Completeness [%]	99.9
Redundancy	9.06
CC(1/2)	0.999	0.878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	2.025 M Ammonium sulfate, 0.1 M Sodium citrate tribasic pH 5.2