7Z1K
Crystal structure of the SPOC domain of human SHARP (SPEN) in complex with RNA polymerase II CTD heptapeptide phosphorylated on Ser5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-13 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9660 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.327, 60.517, 68.273 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.230 - 1.550 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ow1 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.731 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.580 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.072 | |
| Number of reflections | 26393 | 1257 |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 98.8 | |
| Redundancy | 4.3 | |
| CC(1/2) | 0.998 | 0.203 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 0.1 M Potassium thiocyanate, 30% w/v PEG 2000 MME |
