7Z0Q

MHC-II dynamics are maintained in HLA-DR allotypes to ensure catalyzed peptide exchange

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91841
Spacegroup name	H 3
Unit cell lengths	134.094, 134.094, 72.178
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.710 - 2.100
R-factor	0.216
Rwork	0.215
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x5x
RMSD bond length	0.010
RMSD bond angle	0.720
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.710	2.175
High resolution limit [Å]	2.100	2.100
Rmeas	0.076	2.559
Number of reflections	28123	2850
<I/σ(I)>	12.06
Completeness [%]	98.6
Redundancy	4
CC(1/2)	0.999	0.440

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG3350, 0.15M Sodiummalonate