7YZF

Crystal structure of the human FoxA2 bound to the TGTTTATT site (forkhead motif ATAAACA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979260
Spacegroup name	C 2 2 21
Unit cell lengths	45.459, 92.695, 116.842
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.420 - 2.180
R-factor	0.2254
Rwork	0.222
R-free	0.25728
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7yze
RMSD bond length	0.009
RMSD bond angle	1.173
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.421	2.378
High resolution limit [Å]	2.179	2.179
Rpim	0.018	0.544
Number of reflections	9215	462
<I/σ(I)>	19.9
Completeness [%]	86.5	52.2
Redundancy	9.9
CC(1/2)	0.998	0.593

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	25% PEG 3350, 0.1 M Bis-Tris pH 5.5