7YZF
Crystal structure of the human FoxA2 bound to the TGTTTATT site (forkhead motif ATAAACA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979260 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 45.459, 92.695, 116.842 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.420 - 2.180 |
| R-factor | 0.2254 |
| Rwork | 0.222 |
| R-free | 0.25728 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7yze |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.173 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.421 | 2.378 |
| High resolution limit [Å] | 2.179 | 2.179 |
| Rpim | 0.018 | 0.544 |
| Number of reflections | 9215 | 462 |
| <I/σ(I)> | 19.9 | |
| Completeness [%] | 86.5 | 52.2 |
| Redundancy | 9.9 | |
| CC(1/2) | 0.998 | 0.593 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 25% PEG 3350, 0.1 M Bis-Tris pH 5.5 |
