7YYJ
Tetragonal crystal structure of YTHDF1 YTH domain (544AVV546 mutant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 11.2C |
Synchrotron site | ELETTRA |
Beamline | 11.2C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9998 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 120.841, 120.841, 79.636 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.134 - 2.100 |
R-factor | 0.1982 |
Rwork | 0.197 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rci |
RMSD bond length | 0.009 |
RMSD bond angle | 0.935 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 79.640 | 79.640 | 2.160 |
High resolution limit [Å] | 2.100 | 8.910 | 2.100 |
Rmerge | 0.081 | 0.040 | 1.793 |
Rmeas | 0.082 | 0.041 | 1.839 |
Rpim | 0.017 | 0.009 | 0.403 |
Total number of observations | 821804 | 10331 | 58244 |
Number of reflections | 35009 | 554 | 2827 |
<I/σ(I)> | 20.9 | 56.9 | 1.7 |
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 23.5 | 18.6 | 20.6 |
CC(1/2) | 0.999 | 0.999 | 0.756 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 277 | 6-12% PEG3350, 0.2 M KSCN |