7YXP
Crystal structure of WT AncGR2-LBD WT bound to dexamethasone and SHP coregulator fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 178.060, 178.060, 178.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.800 - 3.360 |
| R-factor | 0.2034 |
| Rwork | 0.199 |
| R-free | 0.28820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ufs |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.727 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.800 | 3.630 |
| High resolution limit [Å] | 3.360 | 3.360 |
| Rmeas | 0.108 | |
| Rpim | 0.031 | 0.505 |
| Number of reflections | 7178 | 1431 |
| <I/σ(I)> | 10.8 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.5 | 11.8 |
| CC(1/2) | 1.000 | 0.561 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 0.1 M Tris-HCl, pH 8.0, 0.2 M sodium chloride, 2.0 M ammonium sulfate |
