7YSE
Crystal structure of E. coli heterotetrameric GlyRS in complex with tRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 72.710, 162.123, 324.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 162.120 | 3.060 |
High resolution limit [Å] | 2.907 | 2.907 |
Rmerge | 0.138 | 0.749 |
Rpim | 0.059 | 0.304 |
Number of reflections | 85473 | 12313 |
<I/σ(I)> | 9.4 | |
Completeness [%] | 100.0 | |
Redundancy | 6.6 | |
CC(1/2) | 0.990 | 0.774 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 281 | 0.15M MgCl2, 0.1M NaCl, 0.1M Tris-HCl pH 8.5, 30% PEG 300 |