7YSE

Crystal structure of E. coli heterotetrameric GlyRS in complex with tRNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Glycine--tRNA ligase alpha subunit	polymer	303	34806.3	2	UniProt (P00960) Pfam (PF02091)	Escherichia coli K-12	Glycyl-tRNA synthetase alpha subunit,GlyRS
2	C, D (C, D)	Glycine--tRNA ligase beta subunit	polymer	697	77981.9	2	UniProt (P00961) Pfam (PF02092) Pfam (PF05746)	Escherichia coli K-12	Glycyl-tRNA synthetase beta subunit,GlyRS
3	E, F (E, F)	RNA (76-MER)	polymer	76	24478.5	2	GenBank (1609454293)	Escherichia coli
4	G, I (A, B)	[(2R,3S,4R,5R)-5-(6-azanyl-2-chloranyl-purin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl N-(2-azanylethanoyl)sulfamate	non-polymer		437.8	2	Chemie (JPO)
5	H, J, K, L, M... (A, B, C, D, E)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
6	O, P, Q, R (A, C, D, E)	water	water		18.0	14	Chemie (HOH)

Sequence modifications

C, D: 1 - 689 (UniProt: P00961)

PDB	External Database	Details
Leu 690	-	expression tag
Glu 691	-	expression tag
His 692	-	expression tag
His 693	-	expression tag
His 694	-	expression tag
His 695	-	expression tag
His 696	-	expression tag
His 697	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	274533.4
Non-Polymers*	Number of molecules	8
	Total formula weight	1021.5
All*	Total formula weight	275554.9

*Water molecules are not included.