7YMF

Crystal Structure of DDX3X449_450ET>DP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	H 3
Unit cell lengths	106.560, 106.560, 116.710
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.320 - 2.300
R-factor	0.1881
Rwork	0.185
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pxa
RMSD bond length	0.012
RMSD bond angle	1.375
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX (1.18.1_3865)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.113	0.698
Rpim	0.039	0.264
Number of reflections	21930	2050
<I/σ(I)>	24.5	4.8
Completeness [%]	100.0	99.9
Redundancy	9.2	7.6
CC(1/2)	0.980	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M MES pH 6.5, 1.5 M Sodium Formate , 50 mM Magnesium chloride