7YFF
Structure of GluN1a-GluN2D NMDA receptor in complex with agonist glycine and competitive antagonist CPP.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | DIRECT ELECTRON DE-10 (5k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.6 |
| Number of Particle | 535387 |
| Software | RELION,cryoSPARC,SerialEM,Gctf,UCSF Chimera,PHENIX |
| Symmetry | C2 |
| CTF correction | NONE |
