7YB9

Aspartyl/Asparaginyl beta-hydroxylase (AspH) oxygenase and TPR domains in complex with L-2-hydroxyglutarate and factor X-derived peptide (39mer-4Ser)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97624
Spacegroup name	P 21 21 21
Unit cell lengths	49.672, 91.895, 123.073
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.630 - 1.540
R-factor	0.1857
Rwork	0.185
R-free	0.21470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jtc
RMSD bond length	0.011
RMSD bond angle	1.131
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.900	1.570
High resolution limit [Å]	1.540	1.540
Rmerge	0.075	3.633
Rmeas	0.079	3.797
Rpim	0.022	1.090
Number of reflections	83496	3963
<I/σ(I)>	12.1	0.2
Completeness [%]	99.2	95.5
Redundancy	13.2	11.8
CC(1/2)	0.999	0.356

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.2 M lithium chloride, 20 % w/v Polyethylene glycol 6,000, 0.1 M Bis-Tris propane