7XZ2
TRIM E3 ubiquitin ligase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.001, 326.949, 120.947 |
Unit cell angles | 90.00, 90.89, 90.00 |
Refinement procedure
Resolution | 38.230 - 3.500 |
R-factor | 0.2495 |
Rwork | 0.249 |
R-free | 0.26890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7xv2 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.497 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.560 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 74446 | 3689 |
<I/σ(I)> | 9.32 | |
Completeness [%] | 99.9 | |
Redundancy | 3.8 | |
CC(1/2) | 0.982 | 0.317 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 00 mM Na HEPES NaOH pH 7.5, 230 mM MgCl2, 100 mM CsCl, 34.16% w/v 3/4 EO/OH, Als oil |