7XZ2

TRIM E3 ubiquitin ligase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	77.001, 326.949, 120.947
Unit cell angles	90.00, 90.89, 90.00

Refinement procedure

Resolution	38.230 - 3.500
R-factor	0.2495
Rwork	0.249
R-free	0.26890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xv2
RMSD bond length	0.002
RMSD bond angle	0.497
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.560
High resolution limit [Å]	3.500	3.500
Number of reflections	74446	3689
<I/σ(I)>	9.32
Completeness [%]	99.9
Redundancy	3.8
CC(1/2)	0.982	0.317

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	00 mM Na HEPES NaOH pH 7.5, 230 mM MgCl2, 100 mM CsCl, 34.16% w/v 3/4 EO/OH, Als oil