7XY8

Crystal structure of antibody Fab fragment in complex with CD147(EMMPIRIN)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.980
Spacegroup name	P 1 21 1
Unit cell lengths	64.960, 93.370, 98.309
Unit cell angles	90.00, 90.89, 90.00

Refinement procedure

Resolution	35.910 - 2.300
R-factor	0.238
Rwork	0.236
R-free	0.28090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3b5h
RMSD bond length	0.007
RMSD bond angle	1.304
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.911	2.420
High resolution limit [Å]	2.300	2.300
Rmerge		0.360
Rmeas	0.105	0.439
Rpim	0.060	0.247
Number of reflections	51301	7430
<I/σ(I)>	7.8	2.1
Completeness [%]	98.4	97.8
Redundancy	3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	0.1M Sodium Malonate pH 7.0, 12% (w/v) Polyethylene Glycol 3350