7XUZ
Crystal structure of a HDAC4-MEF2A-DNA ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 65 |
| Unit cell lengths | 93.210, 93.210, 224.920 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.361 - 3.591 |
| R-factor | 0.2605 |
| Rwork | 0.256 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tqe 2h8n |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.450 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.363 | 3.730 |
| High resolution limit [Å] | 3.590 | 3.590 |
| Rmerge | 0.097 | 0.521 |
| Number of reflections | 12749 | 1183 |
| <I/σ(I)> | 19.51 | |
| Completeness [%] | 98.0 | |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0 mM HEPES pH 7.5, 0.2 M NaCl, 3% (v/v) PEG 4000 |
