7XRC
Crystal Structure of the dimeric Brn2 (Pou3f2) POU domain bound to palindromic MORE DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 94.350, 50.630, 69.310 |
Unit cell angles | 90.00, 129.06, 90.00 |
Refinement procedure
Resolution | 34.350 - 1.890 |
R-factor | 0.2014 |
Rwork | 0.200 |
R-free | 0.22680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xsd |
RMSD bond length | 0.008 |
RMSD bond angle | 1.188 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.20.1_4487) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.080 | 2.000 |
High resolution limit [Å] | 1.890 | 1.890 |
Number of reflections | 19759 | 3185 |
<I/σ(I)> | 20.15 | 1.04 |
Completeness [%] | 95.7 | |
Redundancy | 6.96 | |
CC(1/2) | 1.000 | 0.540 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291.15 | 10%PEG 4000,magnesium chloride |