7XPY

Crystal structure of USP7 in complex with its inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97891
Spacegroup name	P 21 21 21
Unit cell lengths	78.005, 80.170, 150.857
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.741 - 2.350
R-factor	0.2261
Rwork	0.224
R-free	0.26580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ylm
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.920	2.430
High resolution limit [Å]	2.350	2.350
Rmeas	0.046	0.869
Number of reflections	40115	3932
<I/σ(I)>	19.5	2.3
Completeness [%]	99.8	100
Redundancy	5.4	5.6
CC(1/2)	0.999	0.832

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	2% v/v Tacsimate pH 7.0, 0.1 M Imidazole pH 7.0, 8% w/v Polyethylene glycol 3350, 5% v/v 2-Propanol