7XL1

Crystal structure of chimeric 7D12-Vob nanobody at 1.65 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-16
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.000
Spacegroup name	P 42 21 2
Unit cell lengths	68.580, 68.580, 103.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.440 - 1.650
R-factor	0.158
Rwork	0.156
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xl0
RMSD bond length	0.007
RMSD bond angle	1.431
Data reduction software	MOSFLM (7.2.2)
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.500	1.740
High resolution limit [Å]	1.650	1.650
Rmerge	0.108	0.963
Rmeas		0.327
Rpim	0.038	0.327
Number of reflections	30042	4233
<I/σ(I)>	12	2.3
Completeness [%]	98.8	97.8
Redundancy	8.7	9.2
CC(1/2)	0.997	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M sodium malonate pH 4.0 20% PEG 3,350