7X8K
Arabidopsis GDP-D-mannose pyrophosphorylase (VTC1) structure (product-bound)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | H 3 2 |
Unit cell lengths | 185.113, 185.113, 371.752 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.820 - 3.000 |
R-factor | 0.2351 |
Rwork | 0.234 |
R-free | 0.26010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7d72 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.091 | |
Rpim | 0.031 | 0.366 |
Number of reflections | 49084 | 4853 |
<I/σ(I)> | 26 | 2.1 |
Completeness [%] | 99.8 | 100 |
Redundancy | 11.1 | 11 |
CC(1/2) | 0.969 | 0.859 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | magnesium formate, PEG 3350 |