7X4N
Crystal Structure of C. elegans kinesin-4 KLP-12 complexed with tubulin and DARPin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2020-10-09 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 82.330, 80.980, 117.750 |
Unit cell angles | 90.00, 93.51, 90.00 |
Refinement procedure
Resolution | 49.250 - 2.880 |
R-factor | 0.2149 |
Rwork | 0.210 |
R-free | 0.29580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mio |
RMSD bond length | 0.010 |
RMSD bond angle | 1.399 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.18.2_3874)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.050 |
High resolution limit [Å] | 2.880 | 2.880 |
Rmeas | 0.355 | 1.970 |
Number of reflections | 34669 | 5455 |
<I/σ(I)> | 19.28 | 3.39 |
Completeness [%] | 98.2 | |
Redundancy | 87.1 | |
CC(1/2) | 0.977 | 0.513 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | ammonium acetate, HEPES, polyethylene glycol (PEG) 3350 |